A protease inhibitor combination therapy under development for the treatment of Sickle Cell Disease, Malaria, and Thrombosis.
Toshihiko Hanada, Maulana A. Empitu, Gregory I. Mines,1 Qianni Ma, Iziegbe L. Omorodion, Ansel Link,1 Christopher J. Schwake, Rachel M. Krueger, Nicholas S. DaRosa, Andrew E. Levin, Edouard Vannier, Athar H. Chishti. Identification of Babesia microti immunoreactive antigens by phage display cDNA screen. Infection and Immunity 2024 Jul 11;92(7):e0021524. https://doi.org/10.1128/iai.00215-24
Christopher J. Schwake, Rachel M. Krueger, Toshihiko Hanada, and Athar H. Chishti. Plasmodium falciparum Glutamic Acid-Rich Protein-Independent Polyclonal Antibodies Inhibit Malaria Parasite Growth in Human Erythrocytes. J Infect Dis. 2024 May 15;229(5):1565-1573.
Daniel I Fritz, Yiwen Ding, Glenn Merrill-Skoloff, Robert Flaumenhaft, Toshihiko Hanada, Athar H Chishti. Dematin Regulates Calcium Mobilization, Thrombosis, and Early Akt Activation in Platelets. Mol Cell Biol. 2023;43(6):283-299. 2023 May 22.
Schwake C, McKay L, Griffiths A, Scartelli C, Flaumenhaft R, Chishti AH. BDA-410 inhibits SARS-CoV-2 main protease activity and viral replication in mammalian cells. J Cell Mol Med. 2022 Sep 9. doi: 10.1111/jcmm.17442. Epub ahead of print. PMID: 36082511.
Schwake C, Hyon M, Chishti AH (2022). Signal peptide peptidase: a potential therapeutic target for parasitic and viral infections. Expert Opinion on Therapeutic Targets. 7:1-13. doi: 10.1080/14728222.2022.2047932. Epub ahead of print. PMID: 35235480.
Mithila F, Schwake C, Fang C, Merrill-Skoloff G, Covic L, Fritz DI, Hanada T, Flaumenhaft R, Chishti AH (2022). Calpain-1 inhibition attenuates in vivo thrombosis in a humanized model of sickle cell disease. Thrombosis Research 4;211:123-126. doi: 10.1016/j. Epub ahead of print. PMID: 35149397.
Almukadi, H., Schwake, C., Kaiser, M., Mayer, G., Schiemer. J., Baldwin, M., Hegde, S., Lu, Y., Hanada. T., and Chishti, A.H. (2019). Human erythrocyte band 3 is a host receptor for Plasmodium falciparum glutamic acid-rich protein. Blood 133:470-480. PMID: 30545833 PMCID: PMC6356982.
Schwake. C., Baldwin., M.R., Bachovchin, W., Hegde, S., Schiemer. J., Okure, C., Levin. A.E., Vannier, E., Hanada, T., and Chishti, A.H. (2019). HIV protease inhibitors block parasite signal peptide peptidases and prevent growth of Babesia microti parasites in erythrocytes. Biochemical and Biophysical Research Communications 517: 125-131.
Fritz, D.I., Hanada, T., Lu, Y., Johnston, J. M., and Chishti, A.H. (2019). MPP1/p55 gene deletion in a hemophilia A patient with ectrodactyly and severe developmental defects. American Journal of Hematology 94: E29-E32. PMID: 30358901.
Nwankwo, J.O., Gremmel, T, Gerrits, A.J., Mithila, F.J., Warburton, R.J., Hill, N.S., Lu, Y., Richey, L.J., Jakubowski, J.A., Frelinger, A.L., and Chishti, A.H. (2017). Calpain-1 regulates platelet function in a humanized mouse model of sickle cell disease. Thrombosis Research 160:58-65. PMID: 29101791 PMCID: PMC5723238.
Nwankwo, J.O., Lei, J., Xu, J., Rivera, A., Gupta, K., Chishti, A.H. (2016). Genetic inactivation of calpain-1 attenuates pain sensitivity in a humanized mouse model of sickle cell disease. Haematologica 101:397-400. PMID: 27418647; PMCID: PMC5046659
Baldwin, M.R., Li, X., Hanada, T., Liu, S-L., and Chishti. A.H. (2015). Merozoite surface protein 1 recognition of host glycophorin A mediates malaria parasite invasion of red blood cells. Blood 125:2704-2711. PMID: 25778531; PMC4408295
Baldwin, M., Yamodo, I., Ranjan, R., Li, X., Mines, G., Marinkovic. M., Hanada, T., Oh, S.S., and Chishti, A.H. (2014). Human erythrocyte Band 3 functions as a receptor for the sialic acid-independent invasion of Plasmodium falciparum. Role of the RhopH3-MSP1 complex. Biochimica et Biophysica Acta-Molecular Cell Research 1843:2855-70. PMID: 25157665
Franceschi, L.D., Franco, R.S., Bertoldi, M., Brugnara, C., Matte, A., Siciliano, A., Wieschhaus, A.J., Chishti, A.H., and Joiner, C.H. (2013). Pharmacological inhibition of calpain-1 prevents red cell dehydration and reduces Gardos channel activity in a mouse model of sickle cell disease. FASEB Journal 27:750-759.
Wieschhaus, A., Khan, A., Zaidi, A., Rogalin, H., Hanada, T., Liu, F., Franceschi, L.D., Brugnara, C., Rivera, A., and Chishti, A.H. (2012). Calpain-1 knockout reveals broad effects on erythrocyte deformability and physiology. Biochemical Journal 448: 141–152.
Kuchay, S.M., Wieschhaus, A.J., Marinkovic, M., Herman, I.M., and Chishti. A.H. (2012). Targeted Gene Inactivation Reveals a Functional Role of Calpain-1 in Platelet Spreading. Journal of Thrombosis and Haemostasis 10:1120-32.
Li, X., Chen, H., Oh, S.S., Chishti, A.H. (2008). A Presenilin-like protease associated with Plasmodium falciparum micronemes is involved in erythrocyte invasion. Molecular Biochemical Parasitology 158:22-31.